4-6 February 2019
Centara Grand at Central Ladprao
Asia/Bangkok timezone
Deadline for full paper revision: 21 May 2019.

68Ga-Labelled Ubiquicidin for Monitoring of Mouse Infected with $\textit{Staphylococcus aureus}$

5 Feb 2019, 14:40
1h 10m
Centara Grand at Central Ladprao

Centara Grand at Central Ladprao

Chatuchak, Bangkok 10900, Thailand
Poster Medical Poster 2

Speaker

Ms Wiranee Sriwiang (Thailand Institute of Nuclear Technology (Public Organisation))

Description

Ubiquicidin is a cationic, antimicrobial peptide found in the innate host defense against pathogens. The peptide is selectively interacted with the surface of microorganism. This work aimed to develop the radiopharmaceutical for infection imaging using antimicrobial peptide. Optimum labeling condition to obtain $^{68}$Ga-DOTA-ubiquicidin (29-41) with radiochemical purity (RCP) more than 90% was evaluated. The RCP was more than 95% with 30 micrograms peptide at solution reaction pH 4. Stability testing of the labeled peptide resembling to in vitro condition using fresh frozen human plasma was studied. The radiolabeled peptide retained its RCP >90% after 4 hours of incubation. Accumulation of $^{68}$Ga-DOTA-UBI at left thigh of mice infected with $\textit{Staphylococcus aureus}$ was greater than that mice inoculated with sterilized S. aureus (inflamed mice) with %ID/g of muscle at 120 minutes were 4.62 ± 3.44 and 1.53 ± 1.73, respectively. These results showed the efficacy of $^{68}$Ga-DOTA-UBI as a radiopharmaceutical for monitoring of bacterial infection.
Keywords: antimicrobial peptide, ubiquicidin, Ga-68

Primary authors

Ms Wiranee Sriwiang (Thailand Institute of Nuclear Technology (Public Organisation)) Ms Wanpen Rangsawai Ms Sudkhanung Pumkhem

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