Curcumin, a naturally derived substance from turmeric, is a well-known topical wound healing agent, which exhibits various properties such as anti-inflammatory, anti-oxidant, and antimicrobial. In this work, we tested the efficacy of crude curcumin prepared from turmeric powder for its wound healing effect in rats. The as-received, commercial Thai turmeric powder was first dissolved in glycerol to make an ointment before sterilization by gamma rays at 25 kGy. HPLC data showed that the curcumin content in irradiated turmeric ointment decreased by about 2.5 folds compared to non-irradiated sample. Full-thickness wounds of size 6 mm in diameter were made surgically on the dorsal side of Sprague Dawley rats, and irradiated turmeric ointment was administered topically. In terms of epithelial healing, wound closure in turmeric-treated rats was observed to be similar to the non-treated group on days 14 and 21 post-wounding. However, histological examination revealed that turmeric better promoted dermal healing as characterized by increasing collagen formation, decreasing granulation tissue, and modulating inflammatory responses especially macrophage and giant cell depositions. Based on these initial results, the use of crude curcumin in irradiated turmeric solution has demonstrated its potential to promote wound healing at the cellular level in animal model.