Roi Et is one of the provinces with rice farming and exported to many countries around the world. In order to assess the radiation risk index from Jasmine rice cultivated soils, 175 samples in Roi Et province were measured using the gamma ray spectrometry technique for estimating the natural radioactivity levels. The specific activity of Ra-226, Th-232 and K-40 was found from 7.14 to 29.02 Bq/Kg with a mean value of 16.92 ± 1.16 Bq/Kg, 7.24 to 33.40 Bq/Kg with a mean value of 16.99 ± 2.24 Bq/Kg and 18.17 to 129.65 Bq/Kg with a mean value of 46.95 ± 6.79 Bq/Kg, respectively. However, the values of radium equivalent activity (Raeq), the external hazard index (Hex), the gamma-absorbed dose rate (D) and the annual effective dose equivalent (AEDEout) were also calculated to evaluate radiological hazards. The average values of Raeq, Hex, D and AEDEout were found to be 44.83 Bq/Kg, 0.12, 20.03 nGy/h and 0.02 mSv/y, respectively. From the results can demonstrate that the natural radioactivity from soil are not affect to population in the area, which the natural radioactivity levels and the radiation risk values of Roi Et soil samples are lower than the UNSCEAR values. Moreover, the data in this research can be used as the basis information of natural background radiation from soil in Roi Et and provides knowledge about the environmental radiation in daily life to people in the area.