Graphene is known to be one of the strongest materials with excellent heat and electricity conductivity that can be used in various applications including solar cells, LED production, and electrical devices. This work aimed to investigate on possible green and environmental-friendly synthesis of reduced graphene oxide (rGO) that can be used as a basis for graphene production using gamma irradiation with the doses varied from 0 to 42 kGy in 7-kGy increments. Analytical techniques used in the characterization of the synthesized rGO included the Scanning Electron Microscopy (SEM) with Energy Dispersive Spectroscopy (EDS), and UV-Vis spectroscopy. The results showed that irradiated graphene oxide (GO) with gamma rays caused the surface of GO to have more wrinkles and crumples due to the formation and reconstruction of new chemical bonds on the single layered carbon, while spectrums from UV-Vis spectroscopy revealed the disappearance of the absorption peak at 292 nm, which indicated n→π* transitions of C=O, after gamma irradiation. Furthermore, results from EDS showed that the ratios of C:O of graphene oxide increased from 49:51 to 69:31 by atomic weight or from 56:44 to 75:25 by atomic contents, after gamma irradiation at 35 kGy. The increases in these reported ratios implied the reduction of oxygen and the production of rGO using gamma irradiation. As a result, gamma irradiation on GO can successfully produce rGO that can be used in graphene production that are much safer and more environment-friendly than using chemical or electrochemical techniques.