INST2019 (International Nuclear Science and Technology Conference)

4-6 February 2019
Asia/Bangkok timezone
Deadline for full paper revision: 21 May 2019.

Method Validation of Tritium Measurement in Urine Sample

4 Feb 2019, 14:50
1h

Chatuchak, Bangkok 10900, Thailand
Poster Physics

Description

Method validation is a key part for radionuclide measurement in biological sample. To confirm that the method used is suitable for the application, this study was designed to validate a H$^{3}$ determination in urine sample using liquid scintillation counting technique. Urine samples obtained from PROCORAD were prepared by mixing 1 ml of urine, 10 ml of Ultima gold cocktail and 4 ml of water; kept in dark for 48 hr and measured for 45 min by Liquid scintillation counter PerkinElmer Tri-Carb 2910 TR. According to the measurement, counting efficiency was determined from quench correction equation. The measurement method was validated by critical limit of detection, minimal detection activity and the recovery percentage of H$^{3}$ in spiked sample. It was found that critical limit of detection and minimal detection activity were 6.47 CPM and 0.96 BqL$^{-1}$ respectively. The recovery of H$^{3}$ spiked sample was in a range of 107.09-111.49% (85-115%: acceptable level). Statistic analysis showed that Z-score is also in a range of 0.03-0.1 (less than 2: satisfactory result). Therefore, this method is suitable for the determination of H$^{3}$ in urine sample.

Keywords: Tritium, Method Validation, Liquid scintillation counter